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SYS · ONLINEPASS · 63.0%
Open Assay
Independent Testing / Est. 2026
BATCH04·26·B
PASS63.0%
N27
PeptidesMetabolicNesiritide

Nesiritide

/ Recombinant 32-residue B-type natriuretic peptide (BNP-32); NPR-A receptor agonist
TIER 1 · ClinicalN = 0 · TESTING PENDINGMW 3464.00 g·mol⁻¹

ALIAS · Natrecor (trade) · Recombinant BNP · BNP · B-type natriuretic peptide

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Research use onlyAny dose figures below describe what specific cited studies used, reported factually. Nothing on this page is guidance for human use.READ FIRST →

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§ A · Identity
Primary sequenceSPKMVQGSGCFGRKMDRISSSSGLGCKVLRRH (disulfide between Cys10 and Cys26)
MW · 3464.00CLASS · Recombinant 32-residue B-type natriuretic peptide (BNP-32); NPR-A receptor agonistCATEGORY · Metabolic

Tier 1 by regulatory status; clinical use has substantially declined. FDA approval 2001 for acutely decompensated heart failure based on the VMAC pivotal trial (NEJM 2002). The ASCEND-HF Phase 4 trial (O'Connor and colleagues, NEJM 2011) randomised approximately 7,141 participants and failed to demonstrate mortality, rehospitalisation, or robust dyspnea benefit, leading to substantial decline in clinical use despite retained approval.

§ B · Mechanism of action

Nesiritide is recombinant human B-type natriuretic peptide (BNP-32), sequence-identical to the endogenous peptide secreted by ventricular cardiomyocytes in response to volume and pressure overload. BNP binds the natriuretic peptide receptor A (NPR-A), a membrane guanylyl cyclase, raising intracellular cGMP and producing vasodilation (arterial and venous), natriuresis, diuresis, and suppression of the renin-angiotensin-aldosterone and sympathetic nervous systems. The acute haemodynamic profile in decompensated heart failure is reduced pulmonary capillary wedge pressure and systemic vascular resistance.

§ C · Human clinical evidence

Tier 1 by trial completeness. The VMAC trial (VMAC Investigators, NEJM 2002) randomised approximately 489 participants with acute decompensated heart failure to nesiritide, nitroglycerin, or placebo, reporting greater pulmonary capillary wedge pressure reduction and dyspnea improvement with nesiritide versus placebo. Subsequent meta-analyses raised concerns about renal and mortality outcomes. The ASCEND-HF Phase 4 trial (O'Connor and colleagues, NEJM 2011) was designed to resolve these signals and randomised approximately 7,141 acutely decompensated heart failure participants; nesiritide produced no mortality benefit, no significant rehospitalisation reduction, and only marginal dyspnea improvement, with a numerical excess of hypotension. The ASCEND-HF result effectively ended the role of nesiritide in routine acute heart failure care.

§ D · Primary literature
PubMed11911755VMAC Investigators et al.Intravenous nesiritide vs nitroglycerin for treatment of decompensated congestive heart failure: a randomized controlled trial · JAMA · human-phase-3-rctIn acute decompensated heart failure, intravenous nesiritide reduced pulmonary capillary wedge pressure and improved dyspnea at three hours versus placebo; modest dyspnea improvement versus nitroglycerin at 24 hours.Limitations: Surrogate endpoints (PCWP, dyspnea) rather than mortality or rehospitalisation; modest sample size relative to subsequent ASCEND-HF.2002
PubMed21732835O'Connor CM et al.Effect of nesiritide in patients with acute decompensated heart failure · The New England Journal of Medicine · human-phase-3-rctIn approximately 7,141 acutely decompensated heart failure participants, nesiritide produced no significant benefit on the co-primary mortality/rehospitalisation endpoint and only marginal dyspnea improvement at 6 and 24 hours; hypotension was more common with nesiritide.Limitations: Phase 4 outcomes trial designed to resolve VMAC-era surrogate-endpoint debate; effectively ended routine clinical use of nesiritide.2011
§ F · Safety signal

Hypotension is the dominant adverse event, present in both VMAC and ASCEND-HF and intrinsic to the vasodilator pharmacology. Renal function decline raised regulatory and clinical concern in the post-VMAC era; ASCEND-HF did not demonstrate a clinically significant worsening renal function signal but did not show benefit either. Rare hypersensitivity reactions and headache have been reported.

§ H · Regulatory status

Regulatory status

FDA status:
FDA-approved
Compounding:
Not eligible for compounding (approved, not in shortage)
§ I · Notable gaps and controversies

Nesiritide is the textbook example of a positive surrogate-endpoint pivotal trial (VMAC: dyspnea, haemodynamics) that did not predict clinical-outcome benefit at scale (ASCEND-HF: mortality, rehospitalisation). The drug remains FDA-approved but is rarely used in current US heart failure practice; guidelines do not recommend it for routine acute decompensated heart failure care. Generic recombinant BNP availability is limited because demand collapsed.